| First Author | Di Lorenzo G | Year | 2018 |
| Journal | Mol Cell Proteomics | Volume | 17 |
| Issue | 8 | Pages | 1612-1626 |
| PubMed ID | 29773673 | Mgi Jnum | J:267919 |
| Mgi Id | MGI:6269084 | Doi | 10.1074/mcp.RA118.000720 |
| Citation | Di Lorenzo G, et al. (2018) Lysosomal Proteome and Secretome Analysis Identifies Missorted Enzymes and Their Nondegraded Substrates in Mucolipidosis III Mouse Cells. Mol Cell Proteomics 17(8):1612-1626 |
| abstractText | Targeting of soluble lysosomal enzymes requires mannose 6-phosphate (M6P) signals whose formation is initiated by the hexameric N-acetylglucosamine (GlcNAc)-1-phosphotransferase complex (alpha2beta2gamma2). Upon proteolytic cleavage by site-1 protease, the alpha/beta-subunit precursor is catalytically activated but the functions of gamma-subunits (Gnptg) in M6P modification of lysosomal enzymes are unknown. To investigate this, we analyzed the Gnptg expression in mouse tissues, primary cultured cells, and in Gnptg reporter mice in vivo, and found high amounts in the brain, eye, kidney, femur, vertebra and fibroblasts. Consecutively we performed comprehensive quantitative lysosomal proteome and M6P secretome analysis in fibroblasts of wild-type and Gnptg(ko) mice mimicking the lysosomal storage disorder mucolipidosis III. Although the cleavage of the alpha/beta-precursor was not affected by Gnptg deficiency, the GlcNAc-1-phosphotransferase activity was significantly reduced. We purified lysosomes and identified 29 soluble lysosomal proteins by SILAC-based mass spectrometry exhibiting differential abundance in Gnptg(ko) fibroblasts which was confirmed by Western blotting and enzymatic activity analysis for selected proteins. A subset of these lysosomal enzymes show also reduced M6P modifications, fail to reach lysosomes and are secreted, among them alpha-l-fucosidase and arylsulfatase B. Low levels of these enzymes correlate with the accumulation of non-degraded fucose-containing glycostructures and sulfated glycosaminoglycans in Gnptg(ko) lysosomes. Incubation of Gnptg(ko) fibroblasts with arylsulfatase B partially rescued glycosaminoglycan storage. Combinatorial treatments with other here identified missorted enzymes of this degradation pathway might further correct glycosaminoglycan accumulation and will provide a useful basis to reveal mechanisms of selective, Gnptg-dependent formation of M6P residues on lysosomal proteins. |
