| First Author | Huang L | Year | 2014 |
| Journal | Nat Commun | Volume | 5 |
| Pages | 5410 | PubMed ID | 25394415 |
| Mgi Jnum | J:223079 | Mgi Id | MGI:5647939 |
| Doi | 10.1038/ncomms6410 | Citation | Huang L, et al. (2014) Decreased tumorigenesis in mice with a Kras point mutation at C118. Nat Commun 5:5410 |
| abstractText | KRAS, NRAS or HRAS genes are mutated to encode an active oncogenic protein in a quarter of human cancers. Redox-dependent reactions can also lead to Ras activation in a manner dependent upon the thiol residue of cysteine 118 (C118). Here, to investigate the effect of mutating this residue on tumorigenesis, we introduce a C118S mutation into the endogenous murine Kras allele and expose the resultant mice to the carcinogen urethane, which induces Kras mutation-positive lung tumours. We report that Kras(+/C118S) and Kras(C118S/C118S) mice develop fewer lung tumours. Although the Kras(C118S) allele does not appear to affect tumorigenesis when the remaining Kras allele is conditionally oncogenic, there is a moderate imbalance of oncogenic mutations favouring the native Kras allele in tumours from Kras(+/C118S) mice treated with urethane. We conclude that the Kras(C118S) allele impedes urethane-induced lung tumorigenesis. |
