| First Author | Yueh WT | Year | 2021 |
| Journal | Development | Volume | 148 |
| Issue | 24 | PubMed ID | 34935904 |
| Mgi Jnum | J:318705 | Mgi Id | MGI:6845038 |
| Doi | 10.1242/dev.199800 | Citation | Yueh WT, et al. (2021) Maternal Smc3 protects the integrity of the zygotic genome through DNA replication and mitosis. Development 148(24):dev199800 |
| abstractText | Aneuploidy is frequently observed in oocytes and early embryos, begging the question of how genome integrity is monitored and preserved during this crucial period. SMC3 is a subunit of the cohesin complex that supports genome integrity, but its role in maintaining the genome during this window of mammalian development is unknown. We discovered that, although depletion of Smc3 following meiotic S phase in mouse oocytes allowed accurate meiotic chromosome segregation, adult females were infertile. We provide evidence that DNA lesions accumulated following S phase in SMC3-deficient zygotes, followed by mitosis with lagging chromosomes, elongated spindles, micronuclei, and arrest at the two-cell stage. Remarkably, although centromeric cohesion was defective, the dosage of SMC3 was sufficient to enable embryogenesis in juvenile mutant females. Our findings suggest that, despite previous reports of aneuploidy in early embryos, chromosome missegregation in zygotes halts embryogenesis at the two-cell stage. Smc3 is a maternal gene with essential functions in the repair of spontaneous damage associated with DNA replication and subsequent chromosome segregation in zygotes, making cohesin a key protector of the zygotic genome. |
