| First Author | Wu H | Year | 2024 |
| Journal | Mol Hum Reprod | Volume | 30 |
| Issue | 8 | PubMed ID | 39037927 |
| Mgi Jnum | J:357575 | Mgi Id | MGI:7734638 |
| Doi | 10.1093/molehr/gaae026 | Citation | Wu H, et al. (2024) Oocyte-specific EXOC5 expression is required for mouse oogenesis and folliculogenesis. Mol Hum Reprod 30(8) |
| abstractText | EXOC5 is a crucial component of a large multi-subunit tethering complex, the exocyst complex, that is required for fusion of secretory vesicles with the plasma membrane. Exoc5 deleted mice die as early embryos. Therefore, to determine the role of EXOC5 in follicular and oocyte development, it was necessary to produce a conditional knockout (cKO), Zp3-Exoc5-cKO, in which Exoc5 was deleted only in oocytes. The first wave of folliculogenesis appeared histologically normal and progressed to the antral stage. However, after IVF with normal sperm, oocytes collected from the first wave (superovulated 21-day-old cKO mice) were shown to be developmentally incompetent. Adult follicular waves did not progress beyond the secondary follicle stage where they underwent apoptosis. Female cKO mice were infertile. Overall, these data suggest that the first wave of folliculogenesis is less sensitive to oocyte-specific loss of Exoc5, but the resulting gametes have reduced developmental competence. In contrast, subsequent waves of folliculogenesis require oocyte-specific Exoc5 for development past the preantral follicle stage. The Zp3-Exoc5-cKO mouse provides a model for disrupting folliculogenesis that also enables the separation between the first and subsequent waves of folliculogenesis. |
