| First Author | Marszalek JR | Year | 2000 |
| Journal | Cell | Volume | 102 |
| Issue | 2 | Pages | 175-87 |
| PubMed ID | 10943838 | Mgi Jnum | J:63427 |
| Mgi Id | MGI:1860998 | Doi | 10.1016/s0092-8674(00)00023-4 |
| Citation | Marszalek JR, et al. (2000) Genetic evidence for selective transport of opsin and arrestin by kinesin-II in mammalian photoreceptors. Cell 102(2):175-87 |
| abstractText | To test whether kinesin-II is important for transport in the mammalian photoreceptor cilium, and to identify its potential cargoes, we used Cre-loxP mutagenesis to remove the kinesin-II subunit, KIF3A, specifically from photoreceptors. Complete loss of KIF3A caused large accumulations of opsin, arrestin, and membranes within the photoreceptor inner segment, while the localization of alpha-transducin was unaffected. Other membrane, organelle, and transport markers, as well as opsin processing appeared normal. Loss of KIF3A ultimately caused apoptotic photoreceptor cell death similar to a known opsin transport mutant. The data suggest that kinesin-II is required to transport opsin and arrestin from the inner to the outer segment and that blocks in this transport pathway lead to photoreceptor cell death as found in retinitis pigmentosa. |
