| First Author | Vacaresse N | Year | 2021 |
| Journal | Open Journal of Immunology | Volume | 11 |
| Pages | 1-24 | Mgi Jnum | J:303166 |
| Mgi Id | MGI:6511944 | Doi | 10.4236/oji.2021.111001 |
| Citation | Vacaresse N, et al. (2021) The Glycosylphosphatidylinositol Anchor Regulates T Cell Antigen Receptor Induced IL-2 Production. Open Journal of Immunology 11:1-24 |
| abstractText | Differential contributions of the glycosylphosphatidylinositol (GPI)-anchor and GPI-anchored proteins (GPI-AP) to signalling remain poorly under-stood. Here we show that GPI-AP deficient murine clones produce on aver-age 18 and 181-fold more IL-2 mRNA and protein, respectively, upon T cell receptor (TCR) stimulation, in a cell-intrinsic fashion. This phenotype is formally attributed to a mutation within the transferase complex that predi-cates the initial step in GPI-anchor biosynthesis. Conditional disruption of the transferase complex enabled the generation of primary GPI-AP deficient CD4+ T cells, which produce on average 10- and 23-fold more IL-2 mRNA and protein, respectively, upon TCR stimulation. Conditional disruption of the transamidase complex yields GPI-sufficient, GPI-AP deficient primary CD4+ T cells. TCR stimulation of these cells yields levels of IL-2 mRNA and protein ranging from 1 - 3 and 3-fold, respectively, of controls. These results provide the first evidence of a profound impact of GPI in the regulation of TCR signalling. |
