| First Author | Yuan X | Year | 2023 |
| Journal | Am J Pathol | Volume | 193 |
| Issue | 4 | Pages | 493-508 |
| PubMed ID | 36638912 | Mgi Jnum | J:340985 |
| Mgi Id | MGI:7463473 | Doi | 10.1016/j.ajpath.2022.12.007 |
| Citation | Yuan X, et al. (2023) Contribution of Hepatic Steatosis-Intensified Extracellular Vesicle Release to Aggravated Inflammatory Endothelial Injury in Liver-Specific Asah1 Gene Knockout Mice. Am J Pathol 193(4):493-508 |
| abstractText | To study the mechanism by which nonalcoholic fatty liver disease (NAFLD) contributes to vascular endothelial Nod-like receptor pyrin domain 3 (NLRP3) inflammasome activation and neointima hyperplasia, NAFLD was established in high-fat diet (HFD)-treated Asah1(fl/fl)/Alb(cre) (liver-specific deletion of the acid ceramidase gene Asah1) mice. Compared with Asah1 flox [Asah1(fl/fl)/wild type (WT)] and wild-type (WT/WT) mice, Asah1(fl/fl)/Alb(cre) mice exhibited significantly enhanced ceramide levels and lipid deposition on HFD in the liver. Moreover, Asah1(fl/fl)/Alb(cre) mice showed enhanced expression of extracellular vesicle (EV) markers, CD63 and annexin II, but attenuated lysosome-multivesicular body fusion. All these changes were accompanied by significantly increased EV counts in the plasma. In a mouse model of neointima hyperplasia, liver-specific deletion of the Asah1 gene enhanced HFD-induced neointima proliferation, which was associated with increased endothelial NLRP3 inflammasome formation and activation and more severe endothelial damage. The EVs isolated from plasma of Asah1(fl/fl)/Alb(cre) mice on HFD were found to markedly enhance NLRP3 inflammasome formation and activation in primary cultures of WT/WT endothelial cells compared with those isolated from WT/WT mice or normal diet-treated Asah1(fl/fl)/Alb(cre) mice. These results suggest that the acid ceramidase/ceramide signaling pathway controls EV release from the liver, and its deficiency aggravates NAFLD and intensifies hepatic EV release into circulation, which promotes endothelial NLRP3 inflammasome activation and consequent neointima hyperplasia in the mouse carotid arteries. |
