| Primary Identifier | MGI:2159651 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Por |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| description | Two mutant cell lines were generated (G1 and H11). While founder mice are indistinguishable H11 contains the desired targeting events while G1 contains Dup(2Csrp2bp-6330439K17Rik)1Bra. This may account for the two types of homozygous mutant types identified. |
| molecularNote | A neomycin resistance gene replaces 2.5 kb of intron 1 and all but the last 6 bp of exon 2. This allele deletes the sequences encoding the translation start site as well as the membrane-binding domain. The targeting event generated a truncated protein which localized to the cytoplasm and not present in microsomes. Cytochrome c reductase activity in liver microsomes isolated from adult heterozygous mice was 50% that found in wild-type mice. |
