| First Author | Chen YT | Year | 2012 |
| Journal | PLoS One | Volume | 7 |
| Issue | 9 | Pages | e46171 |
| PubMed ID | 23049968 | Mgi Jnum | J:191943 |
| Mgi Id | MGI:5463677 | Doi | 10.1371/journal.pone.0046171 |
| Citation | Chen YT, et al. (2012) R26R-GR: a Cre-activable dual fluorescent protein reporter mouse. PLoS One 7(9):e46171 |
| abstractText | Green fluorescent protein (GFP) and its derivatives are the most widely used molecular reporters for live cell imagining. The development of organelle-specific fusion fluorescent proteins improves the labeling resolution to a higher level. Here we generate a R26 dual fluorescent protein reporter mouse, activated by Cre-mediated DNA recombination, labeling target cells with a chromatin-specific enhanced green fluorescence protein (EGFP) and a plasma membrane-anchored monomeric cherry fluorescent protein (mCherry). This dual labeling allows the visualization of mitotic events, cell shapes and intracellular vesicle behaviors. We expect this reporter mouse to have a wide application in developmental biology studies, transplantation experiments as well as cancer/stem cell lineage tracing. |
