| First Author | Ceto S | Year | 2020 |
| Journal | Cell Stem Cell | Volume | 27 |
| Issue | 3 | Pages | 430-440.e5 |
| PubMed ID | 32758426 | Mgi Jnum | J:311408 |
| Mgi Id | MGI:6710949 | Doi | 10.1016/j.stem.2020.07.007 |
| Citation | Ceto S, et al. (2020) Neural Stem Cell Grafts Form Extensive Synaptic Networks that Integrate with Host Circuits after Spinal Cord Injury. Cell Stem Cell 27(3):430-440.e5 |
| abstractText | Neural stem/progenitor cell (NSPC) grafts can integrate into sites of spinal cord injury (SCI) and generate neuronal relays across lesions that can provide functional benefit. To determine if and how grafts become synaptically organized and connect with host systems, we performed calcium imaging of NSPC grafts in SCI sites in vivo and in adult spinal cord slices. NSPC grafts organize into localized and spontaneously active synaptic networks. Optogenetic stimulation of host corticospinal tract axons regenerating into grafts elicited distinct and segregated neuronal network responses throughout the graft. Moreover, optogenetic stimulation of graft-derived axons extending from the graft into the denervated spinal cord also triggered local host neuronal network responses. In vivo imaging revealed that behavioral stimulation likewise elicited focal synaptic responses within grafts. Thus neural progenitor grafts can form functional synaptic subnetworks whose activity patterns resemble intact spinal cord. |
