| First Author | Kim IS | Year | 1999 |
| Journal | Mech Dev | Volume | 80 |
| Issue | 2 | Pages | 159-70 |
| PubMed ID | 10072783 | Mgi Jnum | J:53540 |
| Mgi Id | MGI:1332911 | Doi | 10.1016/s0925-4773(98)00210-x |
| Citation | Kim IS, et al. (1999) Regulation of chondrocyte differentiation by Cbfa1. Mech Dev 80(2):159-70 |
| abstractText | Cbfa1, a developmentally expressed transcription factor of the runt family, was recently shown to be essential for osteoblast differentiation. We have investigated the role of Cbfa1 in endochondral bone formation using Cbfa1-deficient mice. Histology and in situ hybridization with probes for indian hedgehog (Ihh), collagen type X and osteopontin performed at E13.5, E14.5 and E17.5 demonstrated a lack of hypertrophic chondrocytes in the anlagen of the humerus and the phalanges and a delayed onset of hypertrophy in radius/ulna in Cbfa1-/- mice. Detailed analysis of Cbfa1 expression using whole mount in situ hybridization and a lacZ reporter gene reveled strong expression not only in osteoblasts but also in pre-hypertrophic and hypertrophic chondrocytes. Our studies identify Cbfa1 as a major positive regulator of chondrocyte differentiation. Copyright 1998 Elsevier Science Ireland Ltd. |
