Other
17 Authors
- Schwartz RJ,
- Fa'ak F,
- Wijaya CS,
- Azares A,
- Luo W,
- Soibam B,
- Cooney A,
- Liu Y,
- Yu W,
- Benham A,
- Gunaratne P,
- Xu X,
- Stewart MD,
- Lyu Q,
- McConnell BK,
- Chen L,
- Diaz AD
| First Author | Liu Y | Year | 2016 |
| Journal | Sci Rep | Volume | 6 |
| Pages | 31457 | PubMed ID | 27538477 |
| Mgi Jnum | J:259572 | Mgi Id | MGI:6101969 |
| Doi | 10.1038/srep31457 | Citation | Liu Y, et al. (2016) Mesp1 Marked Cardiac Progenitor Cells Repair Infarcted Mouse Hearts. Sci Rep 6:31457 |
| abstractText | Mesp1 directs multipotential cardiovascular cell fates, even though it''s transiently induced prior to the appearance of the cardiac progenitor program. Tracing Mesp1-expressing cells and their progeny allows isolation and characterization of the earliest cardiovascular progenitor cells. Studying the biology of Mesp1-CPCs in cell culture and ischemic disease models is an important initial step toward using them for heart disease treatment. Because of Mesp1''s transitory nature, Mesp1-CPC lineages were traced by following EYFP expression in murine Mesp1(Cre/+); Rosa26(EYFP/+) ES cells. We captured EYFP+ cells that strongly expressed cardiac mesoderm markers and cardiac transcription factors, but not pluripotent or nascent mesoderm markers. BMP2/4 treatment led to the expansion of EYFP+ cells, while Wnt3a and Activin were marginally effective. BMP2/4 exposure readily led EYFP+ cells to endothelial and smooth muscle cells, but inhibition of the canonical Wnt signaling was required to enter the cardiomyocyte fate. Injected mouse pre-contractile Mesp1-EYFP+ CPCs improved the survivability of injured mice and restored the functional performance of infarcted hearts for at least 3 months. Mesp1-EYFP+ cells are bona fide CPCs and they integrated well in infarcted hearts and emerged de novo into terminally differentiated cardiac myocytes, smooth muscle and vascular endothelial cells. |
