| First Author | Ito M | Year | 2005 |
| Journal | Biochem Biophys Res Commun | Volume | 337 |
| Issue | 1 | Pages | 264-70 |
| PubMed ID | 16182245 | Mgi Jnum | J:101519 |
| Mgi Id | MGI:3604222 | Doi | 10.1016/j.bbrc.2005.09.047 |
| Citation | Ito M, et al. (2005) Detection of elements responsible for stage- and tissue-specific expression of mouse Sry using an in vitro Cre/loxP system. Biochem Biophys Res Commun 337(1):264-70 |
| abstractText | We have successfully specified essential sequences of the 5' upstream region for the stage- and tissue-specific expression of mouse Sry by using an in vitro Cre/loxP system. Sry/Cre plasmids carrying Sry 5' sequences of various sizes were transfected into the primary cultured cells from different tissues of CAG/loxP/CAT/loxP/LacZ transgenic fetuses on 11.5-day post coitus (dpc) or 13.5-dpc. Stage- and tissue-specific regulation of Sry expression was disrupted by the deletion of positions 7549-7660 (from -0.4 to -0.5 kb region). In vitro transcription assay also suggested that the region contains element(s) responsible for stage- and tissue-specific expression of mouse Sry. SRY promoter of Shiba goat (Capra hircus var Shiba), a native Japanese miniature goat, showed the tissue-specific activity in the cells from urogenital ridges of the male mouse, but not in the cells from female mice, indicating a possibly different mechanism among species in the regulation of Sry expression. |
