| First Author | Grosjean C | Year | 2021 |
| Journal | STAR Protoc | Volume | 2 |
| Issue | 4 | Pages | 100961 |
| PubMed ID | 34825221 | Mgi Jnum | J:346491 |
| Mgi Id | MGI:7616572 | Doi | 10.1016/j.xpro.2021.100961 |
| Citation | Grosjean C, et al. (2021) Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays. STAR Protoc 2(4):100961 |
| abstractText | Specific antigen recognition by T cell receptor (TCR) activates TCR signaling pathway, leading to T cell proliferation and differentiation into effector and memory cells. Herein, we describe protocols for TCR stimulation assays, including procedures for the isolation and enrichment of mouse splenic T cells for ex vivo TCR stimulation with anti-CD3/CD28 antibodies, and the use of ovalbumin-OT-II mouse model for in vivo TCR stimulation. We applied this protocol to show that MYC protein is essential for T cell proliferation and differentiation. For complete details on the use and execution of this protocol, please refer to Nozais et al. (2021). |
