| First Author | Pankaew S | Year | 2021 |
| Journal | STAR Protoc | Volume | 3 |
| Issue | 1 | Pages | 101041 |
| PubMed ID | 36475567 | Mgi Jnum | J:360184 |
| Mgi Id | MGI:7782686 | Doi | 10.1016/j.xpro.2021.101041 |
| Citation | Pankaew S, et al. (2021) Multiplexed single-cell RNA-sequencing of mouse thymic and splenic samples. STAR Protoc 3(1):101041 |
| abstractText | Multiplexed single-cell RNA-sequencing (scRNA-seq) enables investigating several biological samples in one scRNA-seq experiment. Here, we use antibodies tagged with a hashtag oligonucleotide (Ab-HTO) to label each sample, and 10x Genomics technology to analyze single-cell gene expression. Advantages of sample multiplexing are to reduce the cost of scRNA-seq assay and to avoid batch effect. It may also facilitate cell-doublet removal and the merging of several scRNA-seq assays. Herein, we apply multiplexed scRNA-seq to investigate mouse thymocytes and splenic T lymphocytes development. For complete details on the use and execution of this protocol, please refer to Nozais et al. (2021). |
