| First Author | Imayoshi I | Year | 2013 |
| Journal | Neurosci Res | Volume | 75 |
| Issue | 1 | Pages | 53-8 |
| PubMed ID | 22465523 | Mgi Jnum | J:190172 |
| Mgi Id | MGI:5448340 | Doi | 10.1016/j.neures.2012.03.008 |
| Citation | Imayoshi I, et al. (2013) Light-induced silencing of neural activity in Rosa26 knock-in mice conditionally expressing the microbial halorhodopsin eNpHR2.0. Neurosci Res 75(1):53-8 |
| abstractText | Temporally precise inhibition of genetically defined cell populations in intact nervous systems has been enabled by the microbial halorhodopsin NpHR, a fast, light-activated chloride pump. Here, we report the generation of new mouse strains that express eNpHR2-EYFP fusion proteins after Cre- and/or Flp-mediated recombination to silence neural activity in vivo. In these mouse strains, Cre/Flp recombination induced a high-level of eNpHR2-EYFP expression. Slice whole-cell patch clamp experiments confirmed that eNpHR2-EYFP-expressing neurons could be optically hyperpolarized and inhibited from firing action potentials. Thus, these mouse strains offer powerful tools for light-induced silencing of neural activity in genetically defined cell populations. |
