| Primary Identifier | MGI:2176524 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Ptpn11 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A neomycin resistance cassette replaced a genomic fragment containing exon 3, which contains sequences encoding the SH2-N motif. RT-PCR analysis on total RNA derived from heterozygous mice demonstrated that a transcript was made from this allele that spliced exon 2 to exon 4, resulting in a message that retained the proper reading frame. Western blot analysis on heterozygous specimins confirmed that a mutant protein is expressed from this allele that is consistent with the size expected. |
