| First Author | Mohrs M | Year | 2001 |
| Journal | Immunity | Volume | 15 |
| Issue | 2 | Pages | 303-11 |
| PubMed ID | 11520464 | Mgi Jnum | J:75415 |
| Mgi Id | MGI:2176553 | Doi | 10.1016/s1074-7613(01)00186-8 |
| Citation | Mohrs M, et al. (2001) Analysis of type 2 immunity in vivo with a bicistronic IL-4 reporter. Immunity 15(2):303-11 |
| abstractText | Effector T cells mediate adaptive immunity and immunopathology, but methods for tracking such cells in vivo are limited. We engineered knockin mice expressing IL-4 linked via a viral IRES element with enhanced green fluorescent protein (EGFP). Reporter T cells primed under Th2 conditions showed sensitive and faithful EGFP expression and maintained endogenous IL-4. After Nippostrongylus infection, reporter expression demonstrated the evolution of type 2 immunity from tissue lymphocytes and thence to lymph node CD4(+) T cells, which subsequently migrated into tissue. The appearance of EGFP(+) CD4(+) T cells in tissue, but not in lymph nodes, was Stat6-dependent. Transferred EGFP(+) CD4(+) T cells from infected animals conferred protection against Nippostrongylus to immunodeficient mice. These mice will provide a valuable reagent for assessing immunity in vivo. |
