| First Author | Peter M | Year | 2021 |
| Journal | PLoS One | Volume | 16 |
| Issue | 5 | Pages | e0244038 |
| PubMed ID | 33951054 | Mgi Jnum | J:307127 |
| Mgi Id | MGI:6711756 | Doi | 10.1371/journal.pone.0244038 |
| Citation | Peter M, et al. (2021) Rapid nucleus-scale reorganization of chromatin in neurons enables transcriptional adaptation for memory consolidation. PLoS One 16(5):e0244038 |
| abstractText | The interphase nucleus is functionally organized in active and repressed territories defining the transcriptional status of the cell. However, it remains poorly understood how the nuclear architecture of neurons adapts in response to behaviorally relevant stimuli that trigger fast alterations in gene expression patterns. Imaging of fluorescently tagged nucleosomes revealed that pharmacological manipulation of neuronal activity in vitro and auditory cued fear conditioning in vivo induce nucleus-scale restructuring of chromatin within minutes. Furthermore, the acquisition of auditory fear memory is impaired after infusion of a drug into auditory cortex which blocks chromatin reorganization in vitro. We propose that active chromatin movements at the nucleus scale act together with local gene-specific modifications to enable transcriptional adaptations at fast time scales. Introducing a transgenic mouse line for photolabeling of histones, we extend the realm of systems available for imaging of chromatin dynamics to living animals. |
