| First Author | Hu L | Year | 2014 |
| Journal | Mol Brain | Volume | 7 |
| Pages | 68 | PubMed ID | 25231486 |
| Mgi Jnum | J:232456 | Mgi Id | MGI:5779267 |
| Doi | 10.1186/s13041-014-0068-8 | Citation | Hu L, et al. (2014) A mouse line for inducible and reversible silencing of specific neurons. Mol Brain 7:68 |
| abstractText | BACKGROUND: Genetic methods for inducibly and reversibly inhibiting neuronal activity of specific neurons are critical for exploring the functions of neuronal circuits. The engineered human glycine receptor, called ivermectin (IVM)-gated silencing receptor (IVMR), has been shown to possess this ability in vitro. RESULTS: Here we generated a mouse line, in which the IVMR coding sequence was inserted into the ROSA26 locus downstream of a loxP-flanked STOP cassette. Specific Cre-mediated IVMR expression was revealed by mis-expression of Cre in the striatum and by crossing with several Cre lines. Behavioral alteration was observed in Rosa26-IVMR mice with unilateral striatal Cre expression after systemic administration of IVM, and it could be re-initiated when IVM was applied again. A dramatic reduction in neuron firing was recorded in IVM-treated free moving Rosa26-IVMR;Emx1-Cre mice, and neuronal excitability was reduced within minutes as shown by recording in brain slice. CONCLUSION: This Rosa26-IVMR mouse line provides a powerful tool for exploring selective circuit functions in freely behaving mice. |
