| First Author | Tsukiyama-Kohara K | Year | 2013 |
| Journal | Genes Cells | Volume | 18 |
| Issue | 7 | Pages | 602-7 |
| PubMed ID | 23672244 | Mgi Jnum | J:214044 |
| Mgi Id | MGI:5587899 | Doi | 10.1111/gtc.12059 |
| Citation | Tsukiyama-Kohara K, et al. (2013) 4E-BP1 regulates the differentiation of white adipose tissue. Genes Cells 18(7):602-7 |
| abstractText | 4E Binding protein 1 (4E-BP1) suppresses translation initiation. The absence of 4E-BP1 drastically reduces the amount of adipose tissue in mice. To address the role of 4E-BP1 in adipocyte differentiation, we characterized 4E-BP1(-/-) mice in this study. The lack of 4E-BP1 decreased the amount of white adipose tissue and increased the amount of brown adipose tissue. In 4E-BP1(-/-) MEF cells, PPARgamma coactivator 1 alpha (PGC-1alpha) expression increased and exogenous 4E-BP1 expression suppressed PGC-1alpha expression. The level of 4E-BP1 expression was higher in white adipocytes than in brown adipocytes and showed significantly greater up-regulation in white adipocytes than in brown adipocytes during preadipocyte differentiation into mature adipocytes. The amount of PGC-1alpha was consistently higher in HB cells (a brown preadipocyte cell line) than in HW cells (a white preadipocyte cell line) during differentiation. Moreover, the ectopic over-expression of 4E-BP1 suppressed PGC-1alpha expression in white adipocytes, but not in brown adipocytes. Thus, the results of our study indicate that 4E-BP1 may suppress brown adipocyte differentiation and PGC-1alpha expression in white adipose tissues. |
