| First Author | Hatakeyama J | Year | 2009 |
| Journal | J Dent Res | Volume | 88 |
| Issue | 4 | Pages | 318-22 |
| PubMed ID | 19407150 | Mgi Jnum | J:279218 |
| Mgi Id | MGI:6359118 | Doi | 10.1177/0022034509334749 |
| Citation | Hatakeyama J, et al. (2009) Synergistic roles of amelogenin and ameloblastin. J Dent Res 88(4):318-22 |
| abstractText | Amelogenin and ameloblastin, the major enamel matrix proteins, are important for enamel mineralization. To identify their synergistic roles in enamel development, we generated Amel X(-/-)/Ambn(-/-) mice. These mice showed additional enamel defects in comparison with Amel X(-/-) or Ambn(-/-) mice. In 7-day-old Amel X(-/-)/Ambn(-/-) mice, not only was the ameloblast layer irregular and detached from the enamel surface, as in Ambn(-/-), but also, the enamel width was significantly reduced in the double-null mice as compared with Amel X(-/-) or Ambn(-/-) mice. Proteomic analysis of the double-null teeth revealed increased levels of RhoGDI (Arhgdia), a Rho-family-specific guanine nucleotide dissociation inhibitor, which is involved in important cellular processes, such as cell attachment. Both Amel X(-/-)/Ambn(-/-) mice and Ambn(-/-) mice displayed positive staining with RhoGDI antibody in the irregularly shaped ameloblasts detached from the matrix. Ameloblastin-regulated expression of RhoGDI suggests that Rho-mediated signaling pathway might play a role in enamel formation. |
