| First Author | Baup D | Year | 2010 |
| Journal | Transgenic Res | Volume | 19 |
| Issue | 3 | Pages | 399-414 |
| PubMed ID | 19701794 | Mgi Jnum | J:163584 |
| Mgi Id | MGI:4822322 | Doi | 10.1007/s11248-009-9318-4 |
| Citation | Baup D, et al. (2010) Variegation and silencing in a lentiviral-based murine transgenic model. Transgenic Res 19(3):399-414 |
| abstractText | Lentiviral based constructs represent a recent development in the generation of transgenic animals. The ease of use, and the fact that the same backbone vectors can be used to down-modulate endogenous gene expression and to produce transgenic animals overexpressing a gene of interest, have fuelled growing interest in this technology. In this study, we have used a lentiviral delivery system to generate transgenic mice expressing altered levels (up or downregulated) of a gene of interest. Although this lentiviral-based approach led to high levels of transgenesis and germ line transmission, a wide variation in transgene expression was observed in most first and second generation mouse lines. In particular, despite the segregation of integrants into single-copy expressing mouse lines, transgene expression appeared to be the target of epigenetic regulatory mechanism, often causing the coexistence of high and low transgene expressing cells within a given tissue such as blood peripheral lymphocytes. The establishment and analysis of large number of mouse lines may therefore be required to select a stable transgenic line with pancellular expression of a gene of interest using this lentiviral-based approach. |
