| First Author | Sato M | Year | 2015 |
| Journal | PLoS One | Volume | 10 |
| Issue | 5 | Pages | e0125354 |
| PubMed ID | 25946002 | Mgi Jnum | J:235352 |
| Mgi Id | MGI:5796109 | Doi | 10.1371/journal.pone.0125354 |
| Citation | Sato M, et al. (2015) Generation and Imaging of Transgenic Mice that Express G-CaMP7 under a Tetracycline Response Element. PLoS One 10(5):e0125354 |
| abstractText | The spatiotemporally controlled expression of G-CaMP fluorescent calcium indicator proteins can facilitate reliable imaging of brain circuit activity. Here, we generated a transgenic mouse line that expresses G-CaMP7 under a tetracycline response element. When crossed with a forebrain-specific tetracycline-controlled transactivator driver line, the mice expressed G-CaMP7 in defined cell populations in a tetracycline-controlled manner, notably in pyramidal neurons in layer 2/3 of the cortex and in the CA1 area of the hippocampus; this expression allowed for imaging of the in vivo activity of these circuits. This mouse line thus provides a useful genetic tool for controlled G-CaMP expression in vivo. |
