| First Author | Hou SS | Year | 2023 |
| Journal | Nat Biomed Eng | Volume | 7 |
| Issue | 3 | Pages | 270-280 |
| PubMed ID | 36747008 | Mgi Jnum | J:350674 |
| Mgi Id | MGI:7663181 | Doi | 10.1038/s41551-023-01003-7 |
| Citation | Hou SS, et al. (2023) Near-infrared fluorescence lifetime imaging of amyloid-beta aggregates and tau fibrils through the intact skull of mice. Nat Biomed Eng 7(3):270-280 |
| abstractText | Non-invasive methods for the in vivo detection of hallmarks of Alzheimer's disease can facilitate the study of the progression of the disease in mouse models and may enable its earlier diagnosis in humans. Here we show that the zwitterionic heptamethine fluorophore ZW800-1C, which has peak excitation and emission wavelengths in the near-infrared optical window, binds in vivo and at high contrast to amyloid-beta deposits and to neurofibrillary tangles, and allows for the microscopic imaging of amyloid-beta and tau aggregates through the intact skull of mice. In transgenic mouse models of Alzheimer's disease, we compare the performance of ZW800-1C with that of the two spectrally similar heptamethine fluorophores ZW800-1A and indocyanine green, and show that ZW800-1C undergoes a longer fluorescence-lifetime shift when bound to amyloid-beta and tau aggregates than when circulating in blood vessels. ZW800-1C may prove advantageous for tracking the proteinic aggregates in rodent models of amyloid-beta and tau pathologies. |
