| First Author | Stulić M | Year | 2013 |
| Journal | RNA Biol | Volume | 10 |
| Issue | 10 | Pages | 1611-7 |
| PubMed ID | 24025532 | Mgi Jnum | J:303009 |
| Mgi Id | MGI:6510173 | Doi | 10.4161/rna.26216 |
| Citation | Stulic M, et al. (2013) Spatio-temporal profiling of Filamin A RNA-editing reveals ADAR preferences and high editing levels outside neuronal tissues. RNA Biol 10(10):1611-7 |
| abstractText | RNA editing by ADARs can change the coding potential of protein-coding mRNAs. So far, this type of RNA editing has mainly been shown to affect RNAs expressed in the nervous system with much lower editing levels being observed in other tissues. The actin crosslinking proteins filamin alpha and filamin beta are widely expressed in most tissues. The mRNAs encoding either protein are edited at the same position leading to a conserved Q to R exchange in both proteins. Using bar-coded next generation sequencing, we show that editing of filamin alpha is most abundant in the gastrointestinal tract and only to a lesser extent in the nervous system. Using knockout mice, we show that ADARB1 (ADAR2) is responsible for the majority of FLNA editing, while ADAR1 can edit filamin alpha mRNA in some tissues quite efficiently. Interestingly, editing levels of filamin alpha and beta do not follow the same trend across tissues, suggesting a substrate-specific regulation of editing. |
