| First Author | Borghesi L | Year | 2005 |
| Journal | J Exp Med | Volume | 202 |
| Issue | 12 | Pages | 1669-77 |
| PubMed ID | 16365147 | Mgi Jnum | J:118827 |
| Mgi Id | MGI:3700441 | Doi | 10.1084/jem.20051190 |
| Citation | Borghesi L, et al. (2005) E47 is required for V(D)J recombinase activity in common lymphoid progenitors. J Exp Med 202(12):1669-77 |
| abstractText | Common lymphoid progenitors (CLPs) are the first bone marrow precursors in which V(D)J recombinase activity is up-regulated. Here, we show that loss of the transcription factor E47 produces a reduced CLP population that lacks V(D)J recombinase activity and D-J(H) rearrangements in vivo. Apart from a profound arrest before the pro-B cell stage, other downstream lymphoid progeny of CLPs are still intact in these mice albeit at reduced numbers. In contrast to the inhibition of recombinase activity in early B lineage precursors in E47-deficient animals, loss of either E47 or its cis-acting target Erag (enhancer of rag transcription) has little effect on recombinase activity in thymic T lineage precursors. Taken together, this work defines a role for E47 in regulating lineage progression at the CLP stage in vivo and describes the first transcription factor required for lineage-specific recombinase activity. |
