| First Author | Zhou Q | Year | 2023 |
| Journal | Gastroenterology | Volume | 164 |
| Issue | 4 | Pages | 630-641.e34 |
| PubMed ID | 36623778 | Mgi Jnum | J:336324 |
| Mgi Id | MGI:7441714 | Doi | 10.1053/j.gastro.2022.12.041 |
| Citation | Zhou Q, et al. (2023) Catechol-O-Methyltransferase Loss Drives Cell-Specific Nociceptive Signaling via the Enteric Catechol-O-Methyltransferase/microRNA-155/Tumor Necrosis Factor alpha Axis. Gastroenterology |
| abstractText | BACKGROUND & AIMS: The etiology of abdominal pain in postinfectious, diarrhea-predominant irritable bowel syndrome (PI-IBS-D) is unknown, and few treatment options exist. Catechol-O-methyltransferase (COMT), an enzyme that inactivates and degrades biologically active catecholamines, plays an important role in numerous physiologic processes, including modulation of pain perception. Our objective was to determine the mechanism(s) of how decreased colonic COMT in PI-IBS-D patients contributes to the chronic abdominal pain phenotype after enteric infections. METHODS: Colon neurons, epithelial cells, and macrophages were procured with laser capture microdissection from PI-IBS-D patients to evaluate cell-specific colonic COMT, microRNA-155 (miR-155), and tumor necrosis factor (TNF) alpha expression levels compared to recovered patients (infection cleared: did not develop PI-IBS-D) and control individuals. COMT(-/-), colon-specific COMT(-/-), and miR-155(-/-) mice and human colonoids were used to model phenotypic expression of COMT in PI-IBS-D patients and to investigate signaling pathways linking abdominal pain. Citrobacter rodentium and trinitrobenzene sulfonic acid animal models were used to model postinflammatory changes seen in PI-IBS-D patients. RESULTS: Colonic COMT levels were significantly decreased and correlated with increased visual analog scale abdominal pain ratings in PI-IBS-D patients compared to recovered patients and control individuals. Colonic miR-155 and TNF-alpha were increased in PI-IBS-D patients with diminished colonic COMT. COMT(-/-) mice had significantly increased expression of miR-155 and TNF-alpha in both colon tissues and dorsal root ganglia. Introduction of cV1q antibody (anti-TNF-alpha) into mice reversed visceral hypersensitivity after C rodentium and trinitrobenzene sulfonic acid. CONCLUSIONS: Decreased colonic COMT in PI-IBS-D patients drives abdominal pain phenotypes via the COMT/miR-155/TNF-alpha axis. These important findings will allow new treatment paradigms and more targeted and personalized medicine approaches for gastrointestinal disorders after enteric infections. |
