| First Author | Matsumoto H | Year | 2018 |
| Journal | J Neurosci | Volume | 38 |
| Issue | 41 | Pages | 8745-8758 |
| PubMed ID | 30143574 | Mgi Jnum | J:266383 |
| Mgi Id | MGI:6202812 | Doi | 10.1523/JNEUROSCI.0897-18.2018 |
| Citation | Matsumoto H, et al. (2018) Retinal Detachment-Induced Muller Glial Cell Swelling Activates TRPV4 Ion Channels and Triggers Photoreceptor Death at Body Temperature. J Neurosci 38(41):8745-8758 |
| abstractText | Using region-specific injection of hyaluronic acid, we developed a mouse model of acute retinal detachment (RD) to investigate molecular mechanisms of photoreceptor cell death triggered by RD. We focused on the transient receptor potential vanilloid 4 (TRPV4) ion channel, which functions as a thermosensor, osmosensor, and/or mechanosensor. After RD, the number of apoptotic photoreceptors was reduced by approximately 50% in TRPV4KO mice relative to wild-type mice, indicating the possible involvement of TRPV4 activation in RD-induced photoreceptor cell death. Furthermore, TRPV4 expressed in Muller glial cells can be activated by mechanical stimuli caused by RD-induced swelling of these cells, resulting in release of the cytokine MCP-1, which is reported as a mediator of Muller glia-derived strong mediator for RD-induced photoreceptor death. We also found that the TRPV4 activation by the Muller glial swelling was potentiated by body temperature. Together, our results suggest that RD adversely impacts photoreceptor viability via TRPV4-dependent cytokine release from Muller glial cells and that TRPV4 is part of a novel molecular pathway that could exacerbate the effects of hypoxia on photoreceptor survival after RD.SIGNIFICANCE STATEMENT Identification of the mechanisms of photoreceptor death in retinal detachment is required for establishment of therapeutic targets for preventing loss of visual acuity. In this study, we found that TRPV4 expressed in Muller glial cells can be activated by mechanical stimuli caused by RD-induced swelling of these cells, resulting in release of the cytokine MCP-1, which is reported as a mediator of Muller glia-derived strong mediator for RD-induced photoreceptor death. We also found that the TRPV4 activation by the Muller glial swelling was potentiated by body temperature. Hence, TRPV4 inhibition could suppress cell death in RD pathological conditions and suggests that TRPV4 in Muller glial cells might be a novel therapeutic target for preventing photoreceptor cell death after RD. |
