| First Author | Kretschmer K | Year | 2003 |
| Journal | J Immunol | Volume | 171 |
| Issue | 12 | Pages | 6495-501 |
| PubMed ID | 14662849 | Mgi Jnum | J:87927 |
| Mgi Id | MGI:3028699 | Doi | 10.4049/jimmunol.171.12.6495 |
| Citation | Kretschmer K, et al. (2003) The selection of marginal zone B cells differs from that of B-1a cells. J Immunol 171(12):6495-501 |
| abstractText | Transgenic (Tg) L2 mice expressing high levels of the lambda2 (315) L chain contain only B cell populations involved in the first line of defense, i.e., B-1 and marginal zone (MZ) B cells. The strongly oligoclonal IgH chain repertoire of Tg B-1a cells in such mice was attributed to strong positive selection by autoantigens. In this study, we show that the MZ B cells of L2 mice correspond very closely to MZ B cells of normal mice, as revealed by surface marker expression and gene expression profiling. We demonstrate that the IgH chain repertoire of these Tg MZ B cells is extremely heterogeneous. This is in sharp contrast to the oligoclonality found in B-1a cells of the same mice, which was attributed to strong positive selection mediated by autoantigens. Therefore, the strong positive selection of the IgH chain repertoire in L2 mice is B-1a specific. Thus, our data demonstrate that despite common functional properties, MZ B and B-1a cells exhibit striking differences in their selection and/or maintenance requirements. |
