| First Author | Sakata Y | Year | 2001 |
| Journal | Biochem Biophys Res Commun | Volume | 288 |
| Issue | 4 | Pages | 1032-6 |
| PubMed ID | 11689014 | Mgi Jnum | J:72590 |
| Mgi Id | MGI:2153284 | Doi | 10.1006/bbrc.2001.5871 |
| Citation | Sakata Y, et al. (2001) Analysis of Ca(2+) Currents in Spermatocytes from Mice Lacking Ca(v)2.3 (alpha(1E)) Ca(2+) Channel. Biochem Biophys Res Commun 288(4):1032-6 |
| abstractText | In mammalian male germ-line cells, low-voltage-activated (LVA) Ca(2+) current has been identified and its electrophysiological properties have been studied. To investigate whether alpha(1)2.3 (alpha(1E)) subunit of the voltage-dependent Ca(2+) channel codes for the LVA current, whole-cell patch clamp and following reverse transcription-polymerase chain reaction (RT-PCR) experiments were performed in pachytene spermatocytes from Ca(v)2.3+/+ and Ca(v)2.3-/- mice. Whole-cell current in acutely dissociated pachytene spermatocytes from Ca(v)2.3+/+ and Ca(v)2.3-/- mice displayed a typical profile of LVA Ca(2+) currents and kinetics with no significant differences. Single-cell RT-PCR revealed the expression of Cacna1g in the pachytene spermatocytes from Ca(v)2.3+/+ and Ca(v)2.3-/- mice in which LVA Ca(2+) currents were actually recorded. These results suggest that the Ca(v)2.3 channel makes no detectable contribution to the LVA Ca(2+) current in the pachytene spermatocyte. Instead, Ca(v)3 family such as Ca(v)3.1 may be the likely candidates responsible for the LVA currents in pachytene spermatocytes. Copyright 2001 Academic Press. |
