| First Author | Zizioli D | Year | 1999 |
| Journal | J Biol Chem | Volume | 274 |
| Issue | 9 | Pages | 5385-90 |
| PubMed ID | 10026148 | Mgi Jnum | J:53252 |
| Mgi Id | MGI:1331569 | Doi | 10.1074/jbc.274.9.5385 |
| Citation | Zizioli D, et al. (1999) Early embryonic death of mice deficient in gamma-adaptin. J Biol Chem 274(9):5385-90 |
| abstractText | Intracellular protein transport and sorting by vesicles in the secretory and endocytic pathways requires the formation of a protein coat on the membrane. The heterotetrameric adaptor protein complex 1 (AP-1) promotes the formation of clathrin-coated vesicles at the trans-Golgi network. AP-1 interacts with various sorting signals in the cytoplasmic tails of cargo molecules, thus indicating a function in protein sorting. We generated mutants of the gamma-adaptin subunit of AP-1 in mice to investigate its role in post-Golgi vesicle transport and sorting processes. gamma-Adaptin-deficient embryos develop until day 3.5 post coitus and die during the prenidation period, revealing that AP-1 is essential for viability. In heterozygous mice the amount of AP-1 complexes is reduced to half of controls. Free beta1- or micro1 chains were not detectable, indicating that they are unstable unless they are part of AP-1 complexes. Heterozygous mice weigh less then their wild-type littermates and show impaired T cell development. |
