| First Author | Zhu B | Year | 2023 |
| Journal | Front Physiol | Volume | 14 |
| Pages | 1195001 | PubMed ID | 37324401 |
| Mgi Jnum | J:346938 | Mgi Id | MGI:7490804 |
| Doi | 10.3389/fphys.2023.1195001 | Citation | Zhu B, et al. (2023) Preservation of approximately 12-h ultradian rhythms of gene expression of mRNA and protein metabolism in the absence of canonical circadian clock. Front Physiol 14:1195001 |
| abstractText | Introduction: Besides the approximately 24-h circadian rhythms, approximately 12-h ultradian rhythms of gene expression, metabolism and behaviors exist in animals ranging from crustaceans to mammals. Three major hypotheses were proposed on the origin and mechanisms of regulation of approximately 12-h rhythms, namely, that they are not cell-autonomous and controlled by a combination of the circadian clock and environmental cues, that they are regulated by two anti-phase circadian transcription factors in a cell autonomous manner, or that they are established by a cell-autonomous approximately 12-h oscillator. Methods: To distinguish among these possibilities, we performed a post hoc analysis of two high temporal resolution transcriptome dataset in animals and cells lacking the canonical circadian clock. Results: In both the liver of BMAL1 knockout mice and Drosophila S2 cells, we observed robust and prevalent approximately 12-h rhythms of gene expression enriched in fundamental processes of mRNA and protein metabolism that show large convergence with those identified in wild-type mice liver. Bioinformatics analysis further predicted ELF1 and ATF6B as putative transcription factors regulating the approximately 12-h rhythms of gene expression independently of the circadian clock in both fly and mice. Discussion: These findings provide additional evidence to support the existence of an evolutionarily conserved 12-h oscillator that controls approximately 12-h rhythms of gene expression of protein and mRNA metabolism in multiple species. |
