| First Author | Gross GG | Year | 2016 |
| Journal | Nat Methods | Volume | 13 |
| Issue | 8 | Pages | 673-8 |
| PubMed ID | 27271196 | Mgi Jnum | J:268660 |
| Mgi Id | MGI:6272575 | Doi | 10.1038/nmeth.3894 |
| Citation | Gross GG, et al. (2016) An E3-ligase-based method for ablating inhibitory synapses. Nat Methods 13(8):673-8 |
| abstractText | Although neuronal activity can be modulated using a variety of techniques, there are currently few methods for controlling neuronal connectivity. We introduce a tool (GFE3) that mediates the fast, specific and reversible elimination of inhibitory synaptic inputs onto genetically determined neurons. GFE3 is a fusion between an E3 ligase, which mediates the ubiquitination and rapid degradation of proteins, and a recombinant, antibody-like protein (FingR) that binds to gephyrin. Expression of GFE3 leads to a strong and specific reduction of gephyrin in culture or in vivo and to a substantial decrease in phasic inhibition onto cells that express GFE3. By temporarily expressing GFE3 we showed that inhibitory synapses regrow following ablation. Thus, we have created a simple, reversible method for modulating inhibitory synaptic input onto genetically determined cells. |
