| First Author | Mendoza-Topaz C | Year | 2018 |
| Journal | PLoS One | Volume | 13 |
| Issue | 10 | Pages | e0205306 |
| PubMed ID | 30346954 | Mgi Jnum | J:267005 |
| Mgi Id | MGI:6257953 | Doi | 10.1371/journal.pone.0205306 |
| Citation | Mendoza-Topaz C, et al. (2018) Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix. PLoS One 13(10):e0205306 |
| abstractText | A range of cellular functions have been attributed to caveolae, flask-like invaginations of the plasma membrane. Here, we have used RNA-seq to achieve quantitative transcriptional profiling of primary embryonic fibroblasts from caveolin 1 knockout mice (CAV1-/- MEFs), and thereby to gain hypothesis-free insight into how these cells respond to the absence of caveolae. Components of the extracellular matrix were decisively over-represented within the set of genes displaying altered expression in CAV1-/- MEFs when compared to congenic wild-type controls. This was confirmed biochemically and by imaging for selected examples. Up-regulation of components of the extracellular matrix was also observed in a second cell line, NIH-3T3 cells genome edited to delete CAV1. Up-regulation of components of the extracellular matrix was detected in vivo by assessing collagen deposition and compliance of CAV1-/- lungs. We discuss the implications of these findings in terms of the cellular function of caveolae. |
