| Primary Identifier | MGI:2180366 | Allele Type | Spontaneous |
| Attribute String | Null/knockout | Gene | Art2a |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Sequence analysis of the endogenous loci of C57BL/6 and BALB/c mice revealed 9 nucleotide differences between the two backgrounds. Codon 481 on the C57BL/6 background encodes an in-frame stop signal, putatively precluding the translation of the essential glycosylphosphatidylinositol (GPI) anchor. Protein was undetected by Western blot analysis of COS1 cells transfected with the C57BL/6 variant. The absence of fucntional protein was confirmed by a lack of ADP-ribosyltransferase activity in the COS1 cells. |
