| First Author | Borisovska M | Year | 2005 |
| Journal | EMBO J | Volume | 24 |
| Issue | 12 | Pages | 2114-26 |
| PubMed ID | 15920476 | Mgi Jnum | J:100913 |
| Mgi Id | MGI:3589987 | Doi | 10.1038/sj.emboj.7600696 |
| Citation | Borisovska M, et al. (2005) v-SNAREs control exocytosis of vesicles from priming to fusion. EMBO J 24(12):2114-26 |
| abstractText | SNARE proteins (soluble NSF-attachment protein receptors) are thought to be central components of the exocytotic mechanism in neurosecretory cells, but their precise function remained unclear. Here, we show that each of the vesicle-associated SNARE proteins (v-SNARE) of a chromaffin granule, synaptobrevin II or cellubrevin, is sufficient to support Ca(2+)-dependent exocytosis and to establish a pool of primed, readily releasable vesicles. In the absence of both proteins, secretion is abolished, without affecting biogenesis or docking of granules indicating that v-SNAREs are absolutely required for granule exocytosis. We find that synaptobrevin II and cellubrevin differentially control the pool of readily releasable vesicles and show that the v-SNARE's amino terminus regulates the vesicle's primed state. We demonstrate that dynamics of fusion pore dilation are regulated by v-SNAREs, indicating their action throughout exocytosis from priming to fusion of vesicles. |
