| First Author | Dagenais C | Year | 2000 |
| Journal | J Cereb Blood Flow Metab | Volume | 20 |
| Issue | 2 | Pages | 381-6 |
| PubMed ID | 10698076 | Mgi Jnum | J:61313 |
| Mgi Id | MGI:1354673 | Doi | 10.1097/00004647-200002000-00020 |
| Citation | Dagenais C, et al. (2000) Development of an in situ mouse brain perfusion model and its application to mdr1a P-glycoprotein-deficient mice. J Cereb Blood Flow Metab 20(2):381-6 |
| abstractText | An in situ mouse brain perfusion model predictive of passive and carrier-mediated transport across the blood-brain barrier (BBB) was developed and applied to mdr1a P-glycoprotein (Pgp)-deficient mice [mdr1a(-/-)]. Cerebral flow was estimated from diazepam uptake. Physical integrity of the BBB was assessed with sucrose/inulin spaces; functional integrity was assessed with glucose uptake, which was saturable with a Km of approximately 17 mmol/L and Vmax of 310 mmol x 100 g(-1) x min(-1). Brain uptake of a Pgp substrate (colchicine) was significantly enhanced (two- to fourfold) in mdr1a(-/-) mice. These data suggest that the model is applicable to elucidating the effects of efflux transporters, including Pgp, on brain uptake. |
