| First Author | Sobirin MA | Year | 2012 |
| Journal | Circ Res | Volume | 111 |
| Issue | 8 | Pages | 1037-47 |
| PubMed ID | 22887770 | Mgi Jnum | J:212632 |
| Mgi Id | MGI:5581901 | Doi | 10.1161/CIRCRESAHA.112.270132 |
| Citation | Sobirin MA, et al. (2012) Activation of natural killer T cells ameliorates postinfarct cardiac remodeling and failure in mice. Circ Res 111(8):1037-47 |
| abstractText | RATIONALE: Chronic inflammation in the myocardium is involved in the development of left ventricular (LV) remodeling and failure after myocardial infarction (MI). Invariant natural killer T (iNKT) cells have been shown to produce inflammatory cytokines and orchestrate tissue inflammation. However, no previous studies have determined the pathophysiological role of iNKT cells in post-MI LV remodeling. OBJECTIVE: The purpose of this study was to examine whether the activation of iNKT cells might affect the development of LV remodeling and failure. METHODS AND RESULTS: After creation of MI, mice received the injection of either alpha-galactosylceramide (alphaGC; n=27), the activator of iNKT cells, or phosphate-buffered saline (n=31) 1 and 4 days after surgery, and were followed during 28 days. Survival rate was significantly higher in MI+alphaGC than MI+PBS (59% versus 32%, P<0.05). LV cavity dilatation and dysfunction were significantly attenuated in MI+alphaGC, despite comparable infarct size, accompanied by a decrease in myocyte hypertrophy, interstitial fibrosis, and apoptosis. The infiltration of iNKT cells were increased during early phase in noninfarcted LV from MI and alphaGC further enhanced them. It also enhanced LV interleukin (IL)-10 gene expression at 7 days, which persisted until 28 days. AntienIL-10 receptor antibody abrogated these protective effects of alphaGC on MI remodeling. The administration of alphaGC into iNKT cell-deficient Jalpha18(-/-) mice had no such effects, suggesting that alphaGC was a specific activator of iNKT cells. CONCLUSIONS: iNKT cells play a protective role against post-MI LV remodeling and failure through the enhanced expression of cardioprotective cytokines such as IL-10. |
