| First Author | Kanamori M | Year | 2012 |
| Journal | Int Immunol | Volume | 24 |
| Issue | 2 | Pages | 129-36 |
| PubMed ID | 22247226 | Mgi Jnum | J:182764 |
| Mgi Id | MGI:5316554 | Doi | 10.1093/intimm/dxr108 |
| Citation | Kanamori M, et al. (2012) Sulfatide inhibits alpha-galactosylceramide presentation by dendritic cells. Int Immunol 24(2):129-36 |
| abstractText | Sulfatide-reactive type II NKT cells, the so-called non-invariant NKT (non-iNKT) cells, have been shown to counteract invariant NKT (iNKT) cell activity. However, the effects of sulfatide on activation of iNKT cells by alpha-galactocylceramide (alphaGC) in the context of CD1d have not been studied in detail. Therefore, we studied the blocking effect of sulfatide on alphaGC-induced iNKT cell activation by dendritic cells (DCs). Even in the absence of non-iNKT cells, sulfatide inhibited alphaGC-mediated iNKT cell activation by reducing alphaGC/CD1d complex formations in a dose-dependent manner. This was also confirmed in a cell-free setting using immobilized CD1d-Ig. Moreover, simultaneous injection of alphaGC with sulfatide decreased alphaGC/CD1d complex formations on DCs, accompanied by the reduced CD40L-up-regulation and IFN-gamma production by iNKT cells and IL-12p70 production by DCs. However, sulfatide by itself did not interfere with the presentation of MHC class II-mediated antigen presentation to specific T cells. These results demonstrate that sulfatide competes with alphaGC to be loaded onto CD1d along the endocytic pathway in DCs, thereby inhibiting the iNKT cell response. |
