| First Author | Tang PH | Year | 2017 |
| Journal | Mol Cell Neurosci | Volume | 85 |
| Pages | 70-81 | PubMed ID | 28889993 |
| Mgi Jnum | J:262138 | Mgi Id | MGI:6152366 |
| Doi | 10.1016/j.mcn.2017.09.002 | Citation | Tang PH, et al. (2017) A subpopulation of activated retinal macrophages selectively migrated to regions of cone photoreceptor stress, but had limited effect on cone death in a mouse model for type 2 Leber congenital amaurosis. Mol Cell Neurosci 85:70-81 |
| abstractText | BACKGROUND: Studies of antigen presentation in retina using mice that expressed green fluorescent protein (GFP) from a transgenic CD11c promoter found that retinal GFP(hi) cells possessed antigen presentation function. Subsequent studies found that these high GFP(hi) cells preferentially localized to sites of retinal injury, consistent with their APC function. Interest in the roles of macrophages in degenerative CNS diseases led us to study the GFP(hi) cells in a retinal model of neurodegeneration. We asked if apoptotic cone photoreceptor cell death in Rpe65(-/-) knockout mice induced the GFP(hi) cells, explored their relationship to resident microglia (MG), and tested their role in cone survival. METHODS: Rpe65(-/-) mice were bred to CD11c(GFP) mice on the B6/J background. CD11c(GFP)Rpe65(-/-) mice were also backcrossed to CX3CR1(YFP-creER)ROSA(DTA) mice so that CX3CR1(+) mononuclear cells could be depleted by Tamoxifen. Retinas were analyzed by immunohistochemistry, confocal microscopy, fluorescence fundoscopy and flow cytometry. RESULTS: Elevated numbers of GFP(hi) cells were concentrated in photoreceptor cell layers of CD11c(GFP)Rpe65(-/-) mice coinciding with the peak of cone death at 2 to 4weeks of age, and persisted for at least 14months. After the initial wave of cone loss, a slow progressive loss of cones was found that continued to retain GFP(hi) cells in the outer retina. Sustained, four-week Tamoxifen depletions of the GFP(hi) cells and MG in Rpe65(-/-) mice from day 13 to day 41, and from day 390 to day 420 promoted a small increase in cone survival. We found no evidence that the GFP(hi) cells were recruited from the circulation; all data pointed to a MG origin. MG and GFP(hi) cells were well segregated in the dystrophic retina; GFP(hi) cells were foremost in the photoreceptor cell layer, while MG were concentrated in the inner retina. CONCLUSIONS: The expression of GFP on a subset of retinal mononuclear cells in CD11c(GFP) mice identified a distinct population of cells performing functions previously attributed to MG. Although GFP(hi) cells dominated the macrophage response to cone death in the photoreceptor cell layer, their ablation led to only an incremental increase in cone survival. The ability to identify, ablate, and isolate these cells will facilitate analysis of this activated, antigen-presenting subset of MG. |
