| First Author | Zeng TB | Year | 2021 |
| Journal | Epigenomics | Volume | 13 |
| Issue | 16 | Pages | 1299-1314 |
| PubMed ID | 34519223 | Mgi Jnum | J:356830 |
| Mgi Id | MGI:7762922 | Doi | 10.2217/epi-2021-0168 |
| Citation | Zeng TB, et al. (2021) H3K9 methyltransferase EHMT2/G9a controls ERVK-driven noncanonical imprinted genes. Epigenomics 13(16):1299-1314 |
| abstractText | Aim: Paternal allele-specific expression of noncanonical imprinted genes in the extraembryonic lineages depends on an H3K27me3-based imprint in the oocyte, which is not a lasting mark. We hypothesized that EHMT2, the main euchromatic H3K9 dimethyltransferase, also has a role in controlling noncanonical imprinting. Methods: We carried out allele-specific total RNA-seq analysis in the ectoplacental cone of somite-matched 8.5 days post coitum embryos using reciprocal mouse crosses. Results: We found that the maternal allele of noncanonical imprinted genes was derepressed from its ERVK promoter in the Ehmt2(-/-) ectoplacental cone. In Ehmt2(-/-) embryos, loss of DNA methylation accompanied biallelic derepression of the ERVK promoters. Canonical imprinting and imprinted X chromosome inactivation were generally undisturbed. Conclusion: EHMT2 is essential for repressing the maternal allele in noncanonical imprinting. |
