| First Author | Ribeiro CM | Year | 2017 |
| Journal | Mol Cell Endocrinol | Volume | 442 |
| Pages | 142-152 | PubMed ID | 27989506 |
| Mgi Jnum | J:252821 | Mgi Id | MGI:6095227 |
| Doi | 10.1016/j.mce.2016.12.016 | Citation | Ribeiro CM, et al. (2017) Novel androgen-induced activity of an antimicrobial beta-defensin: Regulation of Wolffian duct morphogenesis. Mol Cell Endocrinol 442:142-152 |
| abstractText | The Wolffian duct (WD) undergoes morphological changes induced by androgens to form the epididymis, which is an organ essential for sperm maturation. Androgen action in WD epithelium involves paracrine factors of mesenchymal origin that function by still poorly understood mechanisms. Here we studied the antimicrobial beta-defensin SPAG11C as a new player in duct morphogenesis, localized prenatally in the WD mesenchyme. Organotypic culture of rat WDs and tissues from Androgen Receptor (AR) knockout mice (ARKO) were used. Our results show that androgen/AR signaling differentially regulated SPAG11C expression at mRNA and protein levels in the developing WD. WDs incubated with recombinant human SPAG11C were shorter and less coiled as a result of reduced epithelial cell proliferation, but not increased apoptosis. Our results suggested beta-defensin SPAG11C as an androgen-target required for WD morphogenesis. This highlights the multifunctional repertoire of the beta-defensin protein family and their potential contribution to the in utero environment that determines male reproductive success. |
