| First Author | Nguyen VTH | Year | 2019 |
| Journal | J Dermatol Sci | Volume | 95 |
| Issue | 1 | Pages | 36-43 |
| PubMed ID | 31262443 | Mgi Jnum | J:289310 |
| Mgi Id | MGI:6434981 | Doi | 10.1016/j.jdermsci.2019.06.005 |
| Citation | Nguyen VTH, et al. (2019) CD22 and CD72 cooperatively contribute to the development of the reverse Arthus reaction model. J Dermatol Sci 95(1):36-43 |
| abstractText | BACKGROUND: Local type III hypersensitivity reactions are acute inflammatory events induced by immune complex (IC) deposition. CD22 and CD72 are B cell-specific cell surface molecules that negatively regulate B cell function. OBJECTIVE: To elucidate the roles of CD22 and CD72 in the development of IgG-mediated type III hypersensitivity reactions. METHOD: The reverse Arthus reaction model in the skin was induced in mice lacking CD22 (CD22(-/-)), CD72 (CD72(-/-)), and both of them (CD22(-/-)/CD72(-/-)). Edema at 4h and hemorrhage at 8h after IC challenge were evaluated. Inflammatory cell infiltration and cytokine and chemokine expression were also examined. RESULTS: Edema and hemorrhage were significantly reduced in CD22(-/-)/CD72(-/-) mice compared with wild-type mice. The loss of both membrane proteins resulted in a greater decrease in edema at 4h, but not hemorrhage at 8h, than the loss of each protein alone. Infiltration of neutrophils, macrophages, and T cells, and the expression of TNF-alpha, IL-6, MIP-1alpha, and CCR5 mRNA were also diminished in the knockout mice compared to wild-type mice, and most significantly reduced in CD22(-/-)/CD72(-/-) mice. Regulatory T (Treg) cells in the spleen were significantly increased in all knockout mice at 4h. Significant differences in the severity of edema and hemorrhage between wild-type and knockout mice were lost when Treg cells were depleted in the knockout mice. CONCLUSION: These results demonstrate that CD22 and CD72 expression contribute to the development of the reverse Arthus reaction model and CD22 and CD72 might be therapeutic targets for human IC-mediated diseases. |
