| Primary Identifier | MGI:2180946 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Prkacb |
| Transmission | Germline | Strain of Origin | 129X1/SvJ |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = REK2 or REK3. |
| molecularNote | A 200 bp region of the gene spanning amino acid 5 of exon 1 to part of intron 1 was replaced with a neomycin resistance cassette via homologous recombination. The gene targeting event specifically disrupts the beta 1 isoform of the gene product. The beta 2 and beta 3 isoforms, which do not utilize exon 1, remain intact. Western blot analysis verified the absence of beta 1 isoform in homozygous mutant animals. |
