| Primary Identifier | MGI:2181054 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Coil |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A 10-kb region spanning exon 2 through the stop codon in exon 7 was replaced by a neomycin selection cassette. The deleted region encoded the nuclear localization signals, serine patches, and a conserved carboxyl terminus. Northern blot analysis using probes corresponding to regions outside of the deleted interval showed the presence of a hybrid mRNA. Sequence analysis of the aberrant transcript showed a cryptic splice junction between exon 1 and the inserted neo gene. Western blot analysis using an antibody raised against the carboxyl terminus showed a lack of normal protein, but could not confirm the absence of an 82 residue peptide putatively translated from the exon 1 fragment. Attempts to generate an antibody for the largely hydrophobic amino terminus were not successful. |
