| First Author | Khan AI | Year | 2013 |
| Journal | Dis Model Mech | Volume | 6 |
| Issue | 4 | Pages | 1021-30 |
| PubMed ID | 23519033 | Mgi Jnum | J:200050 |
| Mgi Id | MGI:5506863 | Doi | 10.1242/dmm.011908 |
| Citation | Khan AI, et al. (2013) Erythropoietin attenuates cardiac dysfunction in experimental sepsis in mice via activation of the beta-common receptor. Dis Model Mech 6(4):1021-30 |
| abstractText | There is limited evidence that the tissue-protective effects of erythropoietin are mediated by a heterocomplex of the erythropoietin receptor and the beta-common receptor ('tissue-protective receptor'), which is pharmacologically distinct from the 'classical' erythropoietin receptor homodimer that is responsible for erythropoiesis. However, the role of the beta-common receptor and/or erythropoietin in sepsis-induced cardiac dysfunction (a well known, serious complication of sepsis) is unknown. Here we report for the first time that the beta-common receptor is essential for the improvements in the impaired systolic contractility afforded by erythropoietin in experimental sepsis. Cardiac function was assessed in vivo (echocardiography) and ex vivo (Langendorff-perfused heart) in wild-type and beta-common receptor knockout mice, that were subjected to lipopolysaccharide (9 mg/kg body weight; young mice) for 16-18 hours or cecal ligation and puncture (aged mice) for 24 hours. Mice received erythropoietin (1000 IU/kg body weight) 1 hour after lipopolysaccharide or cecal ligation and puncture. Erythropoietin reduced the impaired systolic contractility (in vivo and ex vivo) caused by endotoxemia or sepsis in young as well as old wild-type mice in a beta-common-receptor-dependent fashion. Activation by erythropoietin of the beta-common receptor also resulted in the activation of well-known survival pathways (Akt and endothelial nitric oxide synthase) and inhibition of pro-inflammatory pathways (glycogen synthase kinase-3beta, nuclear factor-kappaB and interleukin-1beta). All the above pleiotropic effects of erythropoietin were lost in beta-common receptor knockout mice. Erythropoietin attenuates the impaired systolic contractility associated with sepsis by activation of the beta-common receptor, which, in turn, results in activation of survival pathways and inhibition of inflammation. |
