| Primary Identifier | MGI:2181674 | Allele Type | Targeted |
| Attribute String | Modified regulatory region, Null/knockout | Gene | Nodal |
| Transmission | Germline | Strain of Origin | 129S/SvEv-Gpi1<c> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | By homologous recombination in ES cells heterozygous for the Nodaltm1Rob allele, 100 bp of the ASE enhancer region (including the two 10 bp binding sites) was replaced by a loxP-flanked hygromycin selection cassette in cis to the original mutation. Transient expression of Cre recombinase in targeted ES cells resulted in excision of the selection cassette, leaving a single loxP site in place of the enhancer sequences. |
