| First Author | Marin M | Year | 1997 |
| Journal | Cell | Volume | 89 |
| Issue | 4 | Pages | 619-28 |
| PubMed ID | 9160753 | Mgi Jnum | J:40354 |
| Mgi Id | MGI:87694 | Doi | 10.1016/s0092-8674(00)80243-3 |
| Citation | Marin M, et al. (1997) Transcription factor Sp1 is essential for early embryonic development but dispensable for cell growth and differentiation. Cell 89(4):619-28 |
| abstractText | Transcription factor Sp1 has been implicated in the expression of many genes. Moreover, it has been suggested that Sp1 is linked to the maintenance of methylation-free CpG islands, the cell cycle, and the formation of active chromatin structures. We have inactivated the mouse Sp1 gene. Sp1-/- embryos are retarded in development, show a broad range of abnormalities, and die around day 11 of gestation. In Sp1-/- embryos, the expression of many putative target genes, including cell cycle-regulated genes, is not affected, CpG islands remain methylation free, and active chromatin is formed at the globin loci. However, the expression of the methyl-CpG-binding protein MeCP2 is greatly reduced in Sp1-/- embryos. MeCP2 is thought to be required for the maintenance of differentiated cells. We suggest that Sp1 is an important regulator of this process. |
