| First Author | Zagore LL | Year | 2018 |
| Journal | Cell Rep | Volume | 25 |
| Issue | 5 | Pages | 1225-1240.e6 |
| PubMed ID | 30380414 | Mgi Jnum | J:270404 |
| Mgi Id | MGI:6278458 | Doi | 10.1016/j.celrep.2018.10.012 |
| Citation | Zagore LL, et al. (2018) DAZL Regulates Germ Cell Survival through a Network of PolyA-Proximal mRNA Interactions. Cell Rep 25(5):1225-1240.e6 |
| abstractText | The RNA binding protein DAZL is essential for gametogenesis, but its direct in vivo functions, RNA targets, and the molecular basis for germ cell loss in Dazl-null mice are unknown. Here, we mapped transcriptome-wide DAZL-RNA interactions in vivo, revealing DAZL binding to thousands of mRNAs via polyA-proximal 3' UTR interactions. In parallel, fluorescence-activated cell sorting and RNA-seq identified mRNAs sensitive to DAZL deletion in male germ cells. Despite binding a broad set of mRNAs, integrative analyses indicate that DAZL post-transcriptionally controls only a subset of its mRNA targets, namely those corresponding to a network of genes that are critical for germ cell proliferation and survival. In addition, we provide evidence that polyA sequences have key roles in specifying DAZL-RNA interactions across the transcriptome. Our results reveal a mechanism for DAZL-RNA binding and illustrate that DAZL functions as a master regulator of a post-transcriptional mRNA program essential for germ cell survival. |
